Simple Western Prefilled Chemiluminescence Pipetting Module Protocol

Jess Western Blot Protocol. Jess Automated Western Blot System (004650) by ProteinSimple, Part of Jess also images PVDF or nitrocellulose blotting membranes with proteins transferred from SDS-PAGE gels using traditional Western blotting procedures She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection.

This protocol details an optimized Endo H and PNGase F digestion assay that produces samples that can be run with the JESS Automated Western Blot System. In this application note, we'll walk you through the protocol and show you that protein normalization on Jess is indeed superior to the HKP approach.

Western Blot Process

Just load your samples and reagents into the microplate, and Jess does the rest. Meet Jess, your protein analysis problem solver Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps Find special sample and specific application protocols for these ProteinSimple instruments.

Jess RePlex技术：3μL样品2次连续自动检测实现多重定量Western Blot. On Jess, it's a simple-to-perform added step in a Simple Western size assay Jess Provides Fully Automated Western Blot Analysis ProteinSimple's Jess™ is a Simple Western™ instrument, an innovative technology from Bio-Techne, that performs capillary-based immunoassays and fully automated Western blot analysis, taking the complexity out of traditional immunoassays like Western blot and ELISA and filling in their shortcomings.

Western Blotting Technique Principle Procedure And. She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection steps Find special sample and specific application protocols for these ProteinSimple instruments.